Thirty-Cycle Temperature Optimization of a Closed-Cycle Capillary PCR Machine
نویسندگان
چکیده
منابع مشابه
Thirty-cycle temperature optimization of a closed-cycle capillary PCR machine.
The performance of a novel thermal cycler has been characterized in a 30-cycle PCR. The device consists of a microcapillary equipped with bidirectional pressure-driven flow and in situ optical position sensors. A 1-microL droplet of reaction mixture moves between three heat zones in a 1-mm i.d., oil-filled capillary using a multi-element scattered light detector and active feedback. The design ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2002
ISSN: 0736-6205,1940-9818
DOI: 10.2144/02333st06_11825a